TINAGL1 ELISA Kit (Human) (OKEH07868)

Catalog No: OKEH07868

Size:96T

Human TINAGL1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	93-106% (n = 20)	85-95% (n = 20)	110-120% (n = 20)	100-110% (n = 20)
  EDTA plasma(n=5)	100-109% (n = 20)	105-115% (n = 20)	102-114% (n = 20)	102-112% (n = 20)
  heparin plasma(n=5)	112-122% (n = 20)	105-115% (n = 20)	80-92% (n = 20)	98-108% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology TINAGL1 ELISA Kit (OKEH07868) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-TINAGL1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TINAGL1 and incubated. The TINAGL1 found in the sample or standards competes with the biotinylated TINAGL1 for limited binding sites on the immobilized anti-TINAGL1 antibody. Excess unbound biotinylated TINAGL1 and sample or standard TINAGL1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TINAGL1 captured in the well and inversely proportional to the amount of TINAGL1 which was contained in the sample or standard.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.8% (n = 20)
  Mean Inter-assay CV%: <=9.2% (n = 20)
• ELISA Kit Component:
  • Anti-TINAGL1 Microplate: 96 Wells (12 x 8 Well strips)
  • TINAGL1 Lyophilized Standard: 2 x 100 ng
  • Sample Diluent: 1 x 20 mL
  • 100X TINAGL1-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: May be implicated in the adrenocortical zonation and in mechanisms for repressing the CYP11B1 gene expression in adrenocortical cells. This is a non catalytic peptidase C1 family protein.
  Subcellular Location: Secreted
  Unigene: Hs.199368
  String: 9606.ENSP00000271064
  KEGG: hsa:64129
  MIM: 616064
  Pfam: PF00112
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.34 ng/mL
• Specificity: Natural and recombinant Human Tubulointerstitial nephritis antigen-like
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: TINAGL1
• Gene Full Name: tubulointerstitial nephritis antigen like 1
• Alias Symbols: androgen-regulated gene 1;ARG1;glucocorticoid-inducible protein 5;LCN7;LIECG3;lipocalin 7;OLRG-2;Oxidized LDL-responsive gene 2 protein;oxidized-LDL responsive gene 2;P3ECSL;TIN Ag-related protein;TINAG-like 1;TIN-Ag-RP;TINAGRP;tubulointerstitial nephritis antigen-like;tubulointerstitial nephritis antigen-related protein.
• NCBI Gene Id: 64129
• Protein Name: Q9GZM7
• Description of Target: Tubulointerstitial nephritis antigen-like
• Uniprot ID: https://www.uniprot.org/uniprot/Q9GZM7
• Protein Accession #: NP_001191343.1
• Nucleotide Accession #: NM_001204414.1