TIMP3 Chemi-Luminescent ELISA Kit (Goat) (OKCD03964)

Catalog No: OKCD03964

Size:96WELLS

Goat TIMP3 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Capra hircus|Goat
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-104%	80-89%	96-105%	99-105%
  EDTA Plasma (n=5)	79-103%	79-90%	97-104%	79-94%
  Heparin Plasma (n=5)	97-105%	80-98%	82-95%	80-99%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Tissue Inhibitors Of Metalloproteinase 3 (TIMP3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tissue Inhibitors Of Metalloproteinase 3 (TIMP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) level in the sample or standard.
• ELISA Kit Range: 27.4-20,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	97-105	101
  EDTA Plasma (n=5)	80-89	83
  Heparin Plasma (n=5)	81-92	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Timp3 Microplate: 96 Wells (12 x 8 Well strips)
  • Timp3 Lyophilized Standard: 2
  • 100X Timp3 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
• Sensitivity: 0.312 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TIMP3
• Gene Full Name: TIMP metallopeptidase inhibitor 3
• Alias Symbols: metalloproteinase inhibitor 3;tissue inhibitor of metalloproteinase 3 precursor protein.
• NCBI Gene Id: 102190027
• Protein Name: Tissue inhibitor of metalloproteinase 3 protein
• Uniprot ID: A0A140C4Q7
• Protein Accession #: XP_017903714
• Nucleotide Accession #: XM_018048225