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TIM-1 ELISA Kit (Human) : 96 Wells (OKAG00258)

96 Wells
$419.00
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Predicted Species Reactivity:
Human
Product Format:
1 x 96-Well Plate or 12 x 8-Well Strips
Application:
ELISA
Kit Detection Method:
Colorimetric, OD450 nm
Kit Principle:
The Aviva Human TIM-1 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hTIM-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human TIM-1 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Range:
32-2,000 pg/mL
Kit Component:
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (10.5 ng)
Reconstitution and Storage:
Store as indicated in product manual.
Sample Type:
Cell lysates, sera and plasma
Sensitivity:
32 pg/mL
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
The Human TIM-1 ELISA is capable of recognizing both recombinant and naturally produced Human TIM-1 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: TIM-4
• Murine: TIM-1
• Rat: TIM-1
Assay Info:
Quantitative Colorimentric Sandwich ELISA
Gene Symbol:
HAVCR1
Official Gene Full Name:
hepatitis A virus cellular receptor 1
Alias Symbols:
HAVCR, HAVcr-1, HAVCR-1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, KIM1, KIM-1, T-cell immunoglobulin and mucin domain-containing protein 1, T-cell membrane protein 1, TIM, TIM1, TIM-1, TIMD1, TIMD-1
NCBI Gene Id:
26762
Protein Name:
Hepatitis A virus cellular receptor 1
Description of Target:
The protein encoded by this gene is a membrane receptor for both human hepatitis A virus (HHAV) and TIMD4. The encoded protein may be involved in the moderation of asthma and allergic diseases. The reference genome represents an allele that retains a MTTVP amino acid segment that confers protection against atopy in HHAV seropositive individuals. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 4, 12 and 19.
Swissprot Id:
Q96D42
Protein Accession #:
NP_001166864.1
Nucleotide Accession #:
NM_001173393.2
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express HAVCR1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express HAVCR1.

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