Catalog No: OKCD01082 (Formerly GWB-KBBHM4)
Size:96WELLS
Price: $750.00
SKU
OKCD01082
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Datasheets/ManualsPrintable datasheet for TIGIT ELISA Kit (Human) (OKCD01082)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)78-89%92-101%92-105%89-103%
EDTA plasma(n=5)88-105%79-104%87-96%80-101%
heparin plasma(n=5)78-95%96-103%87-101%78-94%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)78-9186
EDTA plasma(n=5)93-10499
heparin plasma(n=5)92-10197
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-TIGIT Microplate96 Wells (12 x 8 Well Strips)
TIGIT Lyophilized Standard2 x 20 ng
100X Biotinylated TIGIT Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.133 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT). No significant cross-reactivity or interference between T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTIGIT
Gene Full NameT cell immunoreceptor with Ig and ITIM domains
Alias SymbolsT-cell immunoreceptor with Ig and ITIM domains;V-set and immunoglobulin domain containing 9;V-set and immunoglobulin domain-containing protein 9;V-set and transmembrane domain containing 3;V-set and transmembrane domain-containing protein 3;VSIG9;VSIG9, VSTM3;VSTM3;Washington University cell adhesion molecule;WUCAM.
NCBI Gene Id201633
Protein NameT-cell immunoreceptor with Ig and ITIM domains
Description of TargetBinds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Uniprot IDQ495A1
Protein Accession #NP_776160
Nucleotide Accession #NM_173799.3
Protein Size (# AA)244
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