TIA1 Antibody (OAGA04269)

Catalog No: OAGA04269

Size:100 ul

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
diluted at 1:1000

Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TIA1 antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TIA1. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: TIA1
• Gene Full Name: TIA1 cytotoxic granule associated RNA binding protein
• Alias Symbols: WDM, ALS26, TIA-1
• NCBI Gene Id: 7072
• Protein Name: nucleolysin TIA-1 isoform p40; nucleolysin TIA-1
• Description of Target: The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms has been found for this gene.
• Uniprot ID: P31483
• Protein Accession #: NP_071320.2
• Nucleotide Accession #: NM_022037.2