TIA1 Antibody (OACA07277)

Catalog No: OACA07277

Immunohistochemistry of paraffin-embedded human skin at dilution 1:100

Immunohistochemistry of paraffin-embedded human tonsil at dilution 1:100

Immunoprecipitating TIA1 in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG(1ug)instead of OACA09765 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/5,0000)
Lane 2: OACA09765(4ug)+ Hela whole cell lysate(500ug)
Lane 3: Hela whole cell lysate (20ug)

All lanes: Nucleolysin TIA-1 isoform p40 antibody at 2ug/ml
Lane 1: jurkat whole cell lysate
Lane 2: K562 whole cell lysate
Lane 3: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 43,42,25 kDa
Observed band size: 43 kDa

Immunoprecipitating TIA1 in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG(1ug)instead of OACA09765 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/5,0000)
Lane 2: OACA09765(4ug)+ Hela whole cell lysate(500ug)
Lane 3: Hela whole cell lysate (20ug)

All lanes: Nucleolysin TIA-1 isoform p40 antibody at 2ug/ml
Lane 1: jurkat whole cell lysate
Lane 2: K562 whole cell lysate
Lane 3: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 43,42,25 kDa
Observed band size: 43 kDa

Immunohistochemistry of paraffin-embedded human skin at dilution 1:100

Immunohistochemistry of paraffin-embedded human tonsil at dilution 1:100

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry|Immunoprecipitation|Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Nucleolysin TIA-1 isoform p40 protein (1-193AA)
• Purification: Affinity Purified
• Preservative: 0.02% sodium azide
• Formulation: PBS with, 50% glycerol, pH7.3.
• Reference: [1]"Welander distal myopathy caused by an ancient founder mutation in TIA1 associated with perturbed splicing."Klar J., Sobol M., Melberg A., Maebert K., Ameur A., Johansson A.C., Feuk L., Entesarian M., Orlen H., Casar-Borota O., Dahl N.Hum. Mutat. 34:572-577(2013). [2]"Welander distal myopathy is caused by a mutation in the RNA-binding protein TIA1."Hackman P., Sarparanta J., Lehtinen S., Vihola A., Evila A., Jonson P.H., Luque H., Kere J., Screen M., Chinnery P.F., Ahlberg G., Edstrom L., Udd B. Ann. Neurol. 73:500-509(2013). [3]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).

Target Info

• Gene Symbol: TIA1
• Gene Full Name: TIA1 cytotoxic granule associated RNA binding protein
• Alias Symbols: ALS26;nucleolysin TIA-1;nucleolysin TIA-1 isoform p40;p40-TIA-1;p40-TIA-1 (containing p15-TIA-1);RNA-binding protein TIA-1;T-cell-restricted intracellular antigen-1;TIA-1;WDM.
• NCBI Gene Id: 7072
• Protein Name: Nucleolysin TIA-1 isoform p40
• Description of Target: Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
• Uniprot ID: P31483
• Protein Accession #: NP_071320
• Nucleotide Accession #: NM_022037