Catalog No: OKCA00249
Size:96 Wells
Price: $450.00
SKU
OKCA00249
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityCanine
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration< 4 Hours
ELISA Kit PrincipleAviva Systems Biology Thyroxine ELISA Kit (Canine) (OKCA00249) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Canine Thyroxine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Thyroxine and incubated. The Thyroxine found in the sample or standards competes with the biotinylated Thyroxine for limited binding sites on the immobilized anti-Canine Thyroxine antibody. Excess unbound biotinylated Thyroxine and sample or standard Thyroxine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Thyroxine captured in the well and inversely proportional to the amount of Thyroxine which was contained in the sample or standard.
ELISA Kit Range20 ng/mL - 320 ng/mL
ELISA Kit Reproducibility
Mean Intra-Assay Precision %CVMean Inter-Assay Precision %CV
Sample123123
n202020202020

Mean (ng/mL

79.63479.5279.43579.99879.80379.837
SD0.0510.0720.0610.0770.0510.044
CV%5.5747.8496.7028.3725.5844.794
ELISA Kit Component
ComponentAmount
Anti-Thyroxine Microplate96 Well (12 x 8 Well Strips)
Thyroxine Standard 5 x 1 mL
Thyroxine-Biotin Complex1 x 6 mL
Avidin-HRP Conjugate1 x 6 mL
20X Wash Buffer1 x 15 mL
Substrate A1 x 7 mL
Substrate B1 x 7 mL
Stop Solution1 x 7 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell culture supernates, Tissue homogenates
Sensitivity10 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of canine T4.
No significant cross-reactivity or interference between canine T4 and analogues
was observed.
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolThyroxine
Alias SymbolsT4
Protein NameThyroxine
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