SAVE NOW with 10% off all Recombinant Antibodies Shop Now

Catalog No: OKIA00135 (Formerly GWB-790BE4)
Size:1x96WELLPLATE
Price: $577.00
SKU
OKIA00135
Availability: Domestic: within 1 week delivery | International: 1 week
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration100 Minutes
ELISA Kit PrincipleAviva Systems Biology Thrombin III ELISA Kit (Rat) (OKIA00135) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Thrombin III has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Thrombin III - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Thrombin III captured in the well.
ELISA Kit Range12.5 ng/ml - 400 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Rat anti Thrombin III Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Rat anti Thrombin III Calibrator1
Additional InformationIntended Use: The Antithrombin III test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Antithrombin III in biological samples of rats.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Rat Antithrombin III antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3',5,5'-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-RAT ANTITHROMBIN III ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat AT3.
7. RAT ANTITHROMBIN III CALIBRATOR
One vial containing a lyophilized Rat Antithrombin III calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity1.399 ng/ml
Predicted Homology Based on Immunogen SequenceRat
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protein NameThrombin III
Write Your Own Review
You're reviewing:Thrombin III ELISA Kit (Rat) (OKIA00135)
Your Rating