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THRA ELISA Kit (Human) : 96 Wells (OKEH02929)

96 Wells
$529.00
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Application:
ELISA
Gene Symbol:
THRA
Official Gene Full Name:
thyroid hormone receptor, alpha
NCBI Gene Id:
7067
Alias Symbols:
AR7, c-erbA-1, c-ERBA-1, c-erbA-alpha, EAR7, EAR-7, EAR-7.1/EAR-7.2, ERBA, ERBA1, ERB-T-1, MGC000261, MGC43240, NR1A1, Nuclear receptor subfamily 1 group A member 1, THRA1, THRA2, THRA3, Thyroid hormone receptor alpha, V-erbA-related protein 7
Description of Target:
The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Protein Name:
Thyroid hormone receptor alpha
Swissprot Id:
P10827
Protein Accession #:
NP_001177847.1
Nucleotide Accession #:
NM_001190918.1
Predicted Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 0.39 U/L
Kit Range:
1.56 - 100 U/L
Kit Reproducibility:
Mean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology THRA ELISA Kit (Human) (OKEH02929) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for THRA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for THRA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample THRA captured in well.
Kit Component:
ComponentAmount
THRA Microplate96 Wells (12 x 8 Well strips)
THRA Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated THRA Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express THRA.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express THRA.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural human Thyroid hormone receptor alpha. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
55 kDa
Protein Size (# AA):
490

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