THRA ELISA Kit (Human) : 96 Wells (OKEH02929)

Catalog No: OKEH02929

Size:96T

Human THRA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology THRA ELISA Kit (Human) (OKEH02929) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for THRA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for THRA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample THRA captured in well.
• ELISA Kit Range: 1.56-100U/L
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.2% (n = 20)
  Mean Inter-assay CV%: < 10.2% (n = 20)
• ELISA Kit Component:
  • THRA Microplate: 96 Wells (12 x 8 Well strips)
  • THRA Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated THRA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.79U/L
• Specificity: Natural and recombinant Human Thyroid hormone receptor alpha
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: THRA
• Gene Full Name: thyroid hormone receptor alpha
• Alias Symbols: AR7;c-ERBA-1;c-erbA-alpha;CHNG6;EAR7;EAR-7;ERBA;ERBA1;ERBA-related 7;ERB-T-1;NR1A1;nuclear receptor subfamily 1 group A member 1;THRA1;THRA2;thyroid hormone receptor alpha;thyroid hormone receptor alpha 1;thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian);thyroid normone nuclear receptor alpha variant 1;TRalpha;triiodothyronine receptor;V-erbA-related protein 7.
• NCBI Gene Id: 7067
• Protein Name: P10827
• Description of Target: Thyroid hormone receptor alpha
• Uniprot ID: https://www.uniprot.org/uniprot/P10827
• Protein Accession #: NP_001177847.1
• Nucleotide Accession #: NM_001190918.1
• Protein Size (# AA): 490
• Molecular Weight: 55 kDa