THPO ELISA Kit (Dog) (OKEH03956)

Catalog No: OKEH03956

Size:96T

Dog THPO ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Canis lupus, Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology THPO ELISA Kit (Dog) (OKEH03956) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for THPO has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for THPO is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample THPO captured in well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Component:
  • THPO Microplate: 96 Wells (12 x 8 Well strips)
  • THPO Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated THPO Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 11 pg/mL
• Specificity: Natural and recombinant Dog Thrombopoietin
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: THPO
• Gene Full Name: thrombopoietin
• Alias Symbols: C-MPL ligand;Megakaryocyte colony-stimulating factor;Megakaryocyte growth and development factor.
• NCBI Gene Id: 100855822
• Protein Name: P42705
• Description of Target: Thrombopoietin
• Uniprot ID: https://www.uniprot.org/uniprot/P42705
• Protein Accession #: XP_013965923.1
• Nucleotide Accession #: XM_014110448.1
• Protein Size (# AA): 352
• Molecular Weight: 352 kDa