THOC1 Antibody (OAGA08772)

Catalog No: OAGA08772

Size:100 ul

p84 antibody [5E10] detects p84 protein by western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug A431 whole cell lysate/extract
C. 30 ug HeLa whole cell lysate/extract
D. 30 ug HepG2 whole cell lysate/extract
E. 30 ug A375 whole cell lysate/extract
7.5% SDS-PAGE
p84 antibody [5E10] dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Detection of human p84/N5 in nuclear fraction by anti-p84/N5 5E10 monoclonal antibody in western blot experiment. Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.

Sample (50 ug of whole cell lysate)
A: mouse Cerebellum
7.5% SDS PAGE
Diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: HeLa
B: HeLa nucleus
7.5% SDS PAGE
diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
7.5% SDS PAGE
Diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: 293T
B: NIH-3T3
C: mouse brain
D: PC-12
7.5% SDS PAGE
diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

p84 antibody [5E10] detects p84 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p84 protein stained by p84 antibody [5E10] diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.

p84 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 ug of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 ug of p84 antibody [5E10] 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Monkey, Hamster
• Product Format: Liquid
• Clonality: Monoclonal
• Clone: 5E10
• Isotype: IgG2b
• Host: Mouse
• Application: ICC, IF, IHC, IP, WB, CHIP
• Reconstitution and Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.
• Immunogen: Amino acids 15-374 of human p84 expressed in E. coli.
• Purification: Protein G purified
• Concentration: 1 mg/ml
• Application Info: Recommended Starting Dilutions: For ICC/IF: Use at a concentration of 0.5 - 2 ug/ml. For IP: 1:100-1:500. For WB: Use at a concentration of 0.3 - 2 ug/ml. For ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher.
• Storage Buffer: PBS

Target Info

• Gene Symbol: THOC1
• Gene Full Name: THO complex 1
• Alias Symbols: P84, HPR1, P84N5
• NCBI Gene Id: 9984
• Protein Name: THO complex subunit 1
• Description of Target: HPR1 is part of the TREX (transcription/export) complex, which includes TEX1 (MIM 606929), THO2 (MIM 300395), ALY (MIM 604171), and UAP56 (MIM 142560).[supplied by OMIM, Nov 2010]
• Uniprot ID: Q96FV9
• Protein Accession #: NP_005122.2
• Nucleotide Accession #: NM_005131.2