THOC1 Antibody - N-terminal region (OAAB06871)

Catalog No: OAAB06871

Size:400ul

THOC1 Antibody (N-term) (Cat. #OAAB06871) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the THOC1 antibody detected the THOC1 protein (arrow).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Clonality: Monoclonal
• Clone: 301CT40.5.1
• Isotype: IgG3,k
• Host: Mouse
• Application: WB
• Additional Information: Precautions: THOC1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4
  Cellular Location: Isoform 1: Nucleus speckle. Nucleus, nucleoplasm. Nucleus matrix. Cytoplasm. Note=Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution
  Tissue Location: Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This THOC1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 257-285 amino acids from the N-terminal region of human THOC1.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:100~250

Target Info

• Gene Symbol: THOC1
• Alias Symbols: P84, HPR1, P84N5
• NCBI Gene Id: 9984
• Protein Name: THO complex subunit 1
• Description of Target: Mouse Monoclonal Antibody (Mab)
• Uniprot ID: Q96FV9
• Protein Accession #: NP_005122.2
• Molecular Weight: 75666 Da