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Catalog No: OKIA00162 (Formerly GWB-CE987F)
Size:96 Wells
Price: $509.00
SKU
OKIA00162
Availability: Domestic: within 1 week delivery | International: 1 week
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration130 Minutes
ELISA Kit PrincipleAviva Systems Biology Thiostatin ELISA Kit (Rat) (OKIA00162) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for T-kininogen 1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-T-kininogen 1 - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample T-kininogen 1 captured in the well.
ELISA Kit Range6.25 ng/ml - 400 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Rat Thiostatin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Rat Thiostatin Calibrator1
Additional InformationIntended Use: The THIOSTATIN test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring THIOSTATIN in biological samples of rats.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Rat THIOSTATIN antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-RAT THIOSTATIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat Thiostatin.
7. RAT THIOSTATIN CALIBRATOR
One vial containing Rat Thiostatin calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity5.219 ng/ml
Predicted Homology Based on Immunogen SequenceRat
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Alias SymbolsT-kininogen 1, Thiostatin, MAP1, KNG1
Protein NameT-kininogen 1
Description of TargetKininogens are plasma glycoproteins with a number of functions: (1) as precursor of the active peptide bradykinin they effect smooth muscle contraction, induction of hypotension and increase of vascular permeability. (2) They play a role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII. (3) They are inhibitor of thiol proteases.
Uniprot IDP01048
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