THEM2 Antibody (OAPB00922)

Catalog No: OAPB00922

Size:100UG

Western blot analysis of THEM2 in HepG2 cell lysate with THEM2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of THEM2 in human liver tissue with THEM2 antibody at 2.5 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. THEM2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: THEM2 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: THEM2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human THEM2. The immunogen is located within the last 50 amino acids of THEM2.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-THEM2 antibody is Catalog # OEPB03040
• Application Info: THEM2 antibody can be used for detection of THEM2 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Cheng Z, Song F, Shan X, et al. Crystal structure of human thioesterase superfamily member 2. Biochem. Biophys. Res. Commun.2006; 349:172-7.;Wei J, Kang HW, and Cohen DE. Thioesterase superfamily member 2 (Them2)/acyl-CoA thioesterase 13 (Acot13): a homotetrameric hotdog fold thioesterase with selectivity for long-chain fatty acyl-CoAs. Biochem. J.2009; 421:311-22.;Cheng Z, Bao S, Shan X, et al. Human thioesterase superfamily member 2 (hTHEM2) is co-localized with beta-tubulin onto the microtubule. Biochem. Biophys. Res. Commun.2006; 350:850-3.;Scappa EF, Pocai A, Wu MK, et al. Regulation of energy substrate utilization and hepatic insulin sensitivity by phosphatidylcholine transfer protein/StarD. FASEB J.2008; 22:2579-90.

Target Info

• Gene Symbol: ACOT13
• Gene Full Name: acyl-CoA thioesterase 13
• Alias Symbols: acyl-coenzyme A thioesterase 13;hotdog-fold thioesterase superfamily member 2;HT012;hypothalamus protein HT012;palmitoyl-CoA hydrolase;PNAS-27;THEM2;thioesterase superfamily member 2.
• NCBI Gene Id: 55856
• Protein Name: Acyl-coenzyme A thioesterase 13|acyl-coenzyme A thioesterase 13 isoform 1 [Homo sapiens]
• Description of Target: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3-hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro). May play a role in controlling adaptive thermogenesis (By similarity).
• Uniprot ID: Q9NPJ3
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_060943