THBS3 ELISA Kit (Human) (OKCD00691)

Catalog No: OKCD00691 (Formerly GWB-KBBRS5)

Size:96WELLS

Human THBS3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  sodium citrate plasma(n=5)	83-99%	79-90%	95-104%	85-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thrombospondin 3 (THBS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thrombospondin 3 (THBS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thrombospondin 3 (THBS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Thrombospondin 3 (THBS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  sodium citrate plasma(n=5)	78-101	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thrombospondin 3 (THBS3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thrombospondin 3 (THBS3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-THBS3 Microplate: 96 Wells (12 x 8 Well Strips)
  • THBS3 Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated THBS3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Plasma.
• Sensitivity: < 0.61 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Thrombospondin 3 (THBS3). No significant cross-reactivity or interference between Thrombospondin 3 (THBS3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: THBS3
• Gene Full Name: thrombospondin 3
• Alias Symbols: thrombospondin-3;TSP3.
• NCBI Gene Id: 7059
• Protein Name: Thrombospondin-3
• Description of Target: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen.
• Uniprot ID: P49746
• Protein Accession #: NP_001239536
• Nucleotide Accession #: NM_001252607.1
• Protein Size (# AA): 956