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Catalog No: OKEI00185
Size:96 Wells
Price: $625.00
SKU
OKEI00185
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TGTP2 ELISA Kit (Human) (OKEI00185) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TGTP2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TGTP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample TGTP2
ELISA Kit Range1.563 - 100 ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level TGTP2. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level TGTP2 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
TGTP2 Microplate96 Wells (12 x 8 Well strips)
TGTP2 Lyophilized Standard2
100X TGTP2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 10 mL
30X Wash Buffer1 x 30 mL
100X Luminol Substrate1 x 10 mL
Substrate Diluent1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Biological Fluids
Sensitivity0.938 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTGTP2
Alias SymbolsTGTP
NCBI Gene Id100039796
Protein NameT-cell-specific guanine nucleotide triphosphate-binding protein 2
Description of TargetInvolved in innate cell-autonomous resistance to intracellular pathogens, such as Toxoplasma gondii. During avirulent type II T. gondii infection, recruited to the parasitophorous vacuole (PV) membrane, leading to PV vesiculation and rupture, and subsequent digestion of the parasite within the cytosol . Not recruited to virulent type I T. gondii PV membrane . May confer an antiviral state for vesicular stomatitis virus .
Uniprot IDQ3T9E4
Protein Accession #NP_001138636
Nucleotide Accession #NM_001145164
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