Catalog No: OKCD00291 (Formerly GWB-KBBIR3)
Size:96WELLS
Price: $650.00
SKU
OKCD00291
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Datasheets/ManualsPrintable datasheet for TGM1 ELISA Kit (Mouse) (OKCD00291)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)97-104%96-104%80-93%78-94%
EDTA plasma(n=5)81-89%98-105%94-102%87-96%
heparin plasma(n=5)82-90%82-101%96-103%91-98%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transglutaminase 1 (TGM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transglutaminase 1 (TGM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transglutaminase 1 (TGM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transglutaminase 1 (TGM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)79-9791
EDTA plasma(n=5)80-9186
heparin plasma(n=5)92-9996
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transglutaminase 1 (TGM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transglutaminase 1 (TGM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Tgm1 Microplate96 Wells (12 x 8 Well Strips)
Tgm1 Lyophilized Standard2 x 10 ng
100X Biotinylated Tgm1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 0.067 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Transglutaminase 1, Keratinocyte (TGM1). No significant cross-reactivity or interference between Transglutaminase 1, Keratinocyte (TGM1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTgm1
Gene Full Nametransglutaminase 1, K polypeptide
Alias Symbols2310004J08Rik;epidermal TGase;K polypeptide;protein-glutamine gamma-glutamyltransferase K;protein-glutamine-gamma-glutamyltransferase;TG(K);TGas;TGase K;Tgase1;TGase-1;TGK;transglutaminase K;transglutaminase type 1;transglutaminase-1.
NCBI Gene Id21816
Protein NameProtein-glutamine gamma-glutamyltransferase K
Description of TargetCatalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Responsible for cross-linking epidermal proteins during formation of the stratum corneum. Involved in cell proliferation (By similarity).
Uniprot IDQ9JLF6
Protein Accession #NP_001155186
Nucleotide Accession #NM_001161714.1
Protein Size (# AA)815
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