TGFB1 ELISA Kit (Mouse) (OKBB00255)

Catalog No: OKBB00255 (Formerly GWB-ZZD047 )

Size:96 Tests

Mouse TGFB1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's mouse TGF beta 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TGF beta 1 has been precoated onto 96-well plates. Standards(CHO, A279-S390) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF beta 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse TGF beta1 amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	114	240	483	125	248	515
  St. Dev.	5.47	12	17.9	6.75	15.4	30.4
  %CV	4.79	5	3.70	5.4	6.20	5.90

• ELISA Kit Component:
  • Lyophilized recombinant mouse TGFβ1 standard: 10 ng/tube x 2
  • Anti-mouse TGFB1 Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-mouse TGFB1 antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 15.6pg/ml-1000pg/ml
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.1 TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.2 TGFbeta1 is also an important mediator of bone remodeling.3 TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.4 TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion.5 TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein.6 The standard product used in this kit is recombinant TGFβ1 with the molecular mass of 25KDa.
  Activating Reagent: TGFB1 is mostly contained as inactive form in samples, please activate it before assay. Don’t activate recombinant TGFB1.
  Solution A: 1N HCI: add 8.33ml of 12N HCI into 91.67ml of H2O.
  Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H2O, add H2O to adjust volume to 100ml.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum, plasma (EDTA) and urine
• Sensitivity: <2 pg/ml
• Predicted Homology Based on Immunogen Sequence: Cross-reactivates with TGFB2, TGFB3, TGFB5 <1%.
• Reference: 1. Sporn, M. B.; Roberts, A. B.; Wakefield, L. M.; Assoian, R. K. Transforming growth factor-beta: biological function and chemical structure.
  Science 233: 532-534, 1986.
  2. Blanchette, F.; Day, R.; Dong, W.; Laprise, M.-H.; Dubois, C. M. TGF-beta-1 regulates gene expression of its own converting enzyme furin.
  J. Clin. Invest. 99: 1974-1983, 1997.
  3. Janssens, K.; Gershoni-Baruch, R.; Guanabens, N.; Migone, N.; Ralston, S.; Bonduelle, M.; Lissens, W.; Van Maldergem, L.;
  Vanhoenacker, F.; Verbruggen, L.; Van Hul, W. Mutations in the gene encoding the latency-associated peptide of TGF-beta-1 cause
  Camurati-Engelmann disease. Nature Genet. 26: 273-275, 2000.
  4. Li, A. G.; Wang, D.; Feng, X.-H.; Wang, X.-J. Latent TGF-beta-1 overexpression in keratinocytes results in a severe psoriasis-like skin
  disorder. EMBO J. 23: 1770-1781, 2004.
  5. Li, B.; Khanna, A.; Sharma, V.; Singh, T.; Suthanthiran, M.; August, P. TGF-beta-1 DNA polymorphisms, protein levels, and blood pressure.
  Hypertension 33: 271-275, 1999.
  6. Saito, T.; Kinoshita, A.; Yoshiura, K.; Makita, Y.; Wakui, K.; Honke, K.; Niikawa, N.; Taniguchi, N. Domain-specific mutations of a
  transforming growth factor (TGF)-beta 1 latency-associated peptide cause Camurati-Engelmann disease because of the formation of a
  constitutively active form of TGF-beta 1. J. Biol. Chem. 276: 11469-11472, 2001.
• Specificity: Natural and recombinant mouse TGF beta1
• Immunogen: Expression system for standard: CHO; Immunogen sequence: A279-S390
• Description: For quantitative detection of activated mouse TGF beta1 in cell culture supernatants, serum, plasma(EDTA) and urine.

Target Info

• Gene Symbol: TGFB1
• Gene Full Name: transforming growth factor, beta 1
• Alias Symbols: Camurati-Engelmann disease, CED, DPD1, LAP, Latency-associated peptide, TGF beta 1 protein, TGF-beta-1, TGFB, tgfb1, TGFB1_HUMAN, TGFbeta, Transforming growth factor beta 1
• NCBI Gene Id: 21803
• Protein Name: Transforming growth factor beta-1
• Description of Target: Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion.TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein.The standard product used in this kit is recombinant TGFβ1 with the molecular mass of 25KDa.
• Uniprot ID: P04202
• Protein Accession #: NP_035707.1
• Nucleotide Accession #: NM_011577.2