Tgfb3 ELISA Kit (Rat) (OKEH04568)

Catalog No: OKEH04568

Size:96T

Rat Tgfb3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Tgfb3 ELISA Kit (Rat) (OKEH04568) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tgfb3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Tgfb3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tgfb3 captured in well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Anti-Tgfb3 Microplate: 96 Wells (12 x 8 Well Strips)
  • Tgfb3 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • Tgfb3 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 8.01 pg/mL
• Specificity: Natural and recombinant Rat Transforming growth factor beta-3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Tgfb3
• Gene Full Name: transforming growth factor, beta 3
• Alias Symbols: TGF-B3;TGF-beta-3;transforming growth factor beta-3 proprotein.
• NCBI Gene Id: 25717
• Protein Name: Q07258
• Description of Target: Transforming growth factor beta-3
• Uniprot ID: https://www.uniprot.org/uniprot/Q07258
• Protein Accession #: NP_037306.1
• Nucleotide Accession #: NM_013174.2
• Protein Size (# AA): 412
• Molecular Weight: 47 KDa