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Catalog No: OKCD07828
Size:96WELLS
Price: $650.00
SKU
OKCD07828
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Datasheets/ManualsPrintable datasheet for TGFB3 ELISA Kit (Mouse) (OKCD07828)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 3 (TGFb3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 3 (TGFb3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta 3 (TGFb3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transforming Growth Factor Beta 3 (TGFb3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta 3 (TGFb3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 3 (TGFb3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
TGFB3 Microplate96 Wells (12 x 8 Well strips)
TGFB3 Lyophilized Standard2
100X TGFB3 HRP-Detector Anitbody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sensitivity< 2.9pg/mL
Gene SymbolTGFB3
Gene Full Nametransforming growth factor beta 3
Alias SymbolsTGF-B3; LAP; Latency-associated peptide
Protein NameTransforming growth factor beta-3
Description of TargetInvolved in embryogenesis and cell differentiation.
Uniprot IDP17125
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