Catalog No: OKEH04520
Size:96T
Price: $425.00
SKU
OKEH04520
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Tgfb2 ELISA Kit (Rat) (OKEH04520) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tgfb2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Tgfb2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tgfb2 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-Tgfb2 Microplate96 Wells (12 x 8 Well Strips)
Tgfb2 Lyophilized Standard2
Sample Diluent1 x 20 mL
Tgfb2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity7.83 pg/mL
SpecificityNatural and recombinant Rat Transforming growth factor beta-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTgfb2
Gene Full Nametransforming growth factor, beta 2
Alias SymbolsTGF beta 2 protein;TGF-B2;TGF-beta-2;transforming growth factor beta-2 proprotein.
NCBI Gene Id81809
Protein NameQ07257
Description of TargetTransforming growth factor beta-2
Uniprot IDhttps://www.uniprot.org/uniprot/Q07257
Protein Accession #NP_112393.1
Nucleotide Accession #NM_031131.1
Protein Size (# AA)442
Molecular Weight51 KDa
Write Your Own Review
You're reviewing:Tgfb2 ELISA Kit (Rat) (OKEH04520)
Your Rating
We found other products you might like!