TGFB1 ELISA Kit (Sheep) (OKCA01965)

Catalog No: OKCA01965

Size:96 Wells

Sheep TGFB1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Sheep
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution

  Mean Recovery (%)

  Range (%)

• ELISA Kit Principle: Aviva Systems Biology TGFB1 ELISA Kit (Sheep) (OKCA01965) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TGFB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TGFB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample TGFB1 captured in the well.
• ELISA Kit Range: 15.6 - 1,000 ng/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	96	89-101
  EDTA Plasma (n=4)	97	87-105

• ELISA Kit Component:
  • TGFB1 Microplate: 96 Wells (12 x 8 Well strips)
  • TGFB1 Lyophilized Standard: 2 x 1 ug
  • 100X Biotinylated TGFB1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma
• Sensitivity: 3.9 ng/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: TGFB1
• Gene Full Name: Transforming growth factor beta-1
• Alias Symbols: CED, DPD1, LAP, TGFB, TGFbeta, TGF-beta 1 protein|latency-associated peptide
• NCBI Gene Id: 443417
• Protein Name: Transforming growth factor beta-1
• Description of Target: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP). Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types.
• Uniprot ID: P50414
• Protein Accession #: NP_001009400.1
• Nucleotide Accession #: NM_001009400.1