TGFB1 ELISA Kit (Rabbit) (OKBB00746)

Catalog No: OKBB00746

Size:96 wells

TGFB1 ELISA Kit (Rabbit) (OKBB00746) Standard Curve

Product Info

• Predicted Species Reactivity: Rabbit
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's Rabbit TGF beta 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF beta 1 has been precoated onto 96-well plates. Standards(CHO, A279-S390) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF beta 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Rabbit TGF beta 1 amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	135	264	521	152	270	543
  Standard deviation	3.64	13.73	18.8	7.6	17.3	32.04
  CV(%)	4.4	5.2	3.6	5	6.4	5.9

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Rabbit TGFB1 Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Rabbit TGFB1 Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Rabbit TGFB1 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	30	29	32	28	29	1.47	5%
  Sample 2	156	166	150	158	157	5.72	3.6%
  Sample 3	497	448	471	476	473	17.42	3.6%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
  O.D.	0.039	0.108	0.186	0.321	0.599	1.017	1.586	2.200

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated rabbit TGF beta 1
• Sensitivity: <1 pg/ml
• Immunogen: Expression system for standard: CHO; Immunogen sequence: A279-S390
• Description: Sandwich high sensitivity ELISA kit for Quantitative Detection of activated rabbit TGF beta 1. 96wells/kit, with removable strips.

Target Info

• Gene Symbol: TGFB1
• Gene Full Name: Transforming growth factor beta-1
• Alias Symbols: Transforming growth factor beta-1;TGF-beta-1;Latency-associated peptide;LAP;TGFB1;TGFB;
• NCBI Gene Id: 100008645
• Protein Name: Transforming growth factor beta-1
• Description of Target: Transforming growth factor-beta1(TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide(beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.
• Uniprot ID: P01137
• Protein Accession #: NP_000651.3
• Nucleotide Accession #: NM_000660.6