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Catalog No: OKBB00919
Size:96 wells
Price: $538.00
SKU
OKBB00919
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's human LAP(TGF-beta1) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LAP(TGF-beta1) has been precoated onto 96-well plates. Standards(NS0, L30-R278) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LAP(TGF-beta1) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human LAP(TGF-beta1) amount of sample captured in plate.
ELISA Kit Range62.5 pg/ml - 4,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)876732000906982085
Standard deviation3.9143.741264.557.93145.95
CV(%)4.56.56.358.37
ELISA Kit Component
ComponentAmount
96-Well Plate Pre-coated with Anti-Human TGFB1 Antibody96 Wells (12 x 8 Well Strips)
Lyophilized Recombinant Human TGFB1 Standard2 x 10 ng
100X Biotinylated Anti-Human TGFB1 Antibody1 x 130 uL
100X Avidin-Biotin Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Additional Information

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 18710488101957.587.9%
Sample 267368461972167436.515.4%
Sample 320001784201117991898107.25.6%

*number of samples for each test n=16.

::
Standard Number87654321
Concentration (pg/ml)062.5125250500100020004000
O.D.0.0450.1190.1430.3080.5060.9311.6272.158
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample Typecell culture supernates,cell lysates, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human LAP(TGF-beta1)
ImmunogenExpression system for standard: NS0; Immunogen sequence: L30-R278
DescriptionFor quantitative detection of human LAP(TGF-beta1) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolTGFB1
Gene Full Nametransforming growth factor, beta 1
Alias SymbolsCamurati-Engelmann disease, CED, DPD1, LAP, Latency-associated peptide, TGF beta 1 protein, TGF-beta-1, TGFB, tgfb1, TGFB1_HUMAN, TGFbeta, Transforming growth factor beta 1
NCBI Gene Id7939
Protein NameTransforming growth factor beta-1
Description of TargetTGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide(beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. Processing and cleavage of the precursor protein between amino acids 278 and 279 results in the formation of LAP dimers and TGF beta dimers that then non-covalently associate with each other to form the small latent TGF beta complex. LAP is secreted and can be found in the extracellular matrix. In addition, LAP can also be expressed on platelets and activated regulatory T cells.
Uniprot IDP01137
Protein Accession #NP_000651.3
Nucleotide Accession #NM_000660.6
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