- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for anti-TGFB1 (OALA04428) antibody |
|---|
| Tested Species Reactivity | Human |
|---|---|
| Predicted Species Reactivity | Human |
| Product Format | Liquid |
| Clonality | Monoclonal |
| Clone | TB21 |
| Isotype | IgG1,k |
| Host | Mouse |
| Conjugation | Unconjugated |
| Application | ELISA, FN, IHC, IHC-F, IHC-P, NT, WB |
| Reconstitution and Storage | Short term: store at 4C. Long term: aliquot and store at -20C. Avoid freeze-thaw cycles. |
| Immunogen | TGF-b from human platelets. |
| Purification | Protein G purified |
| Concentration | 1 mg/ml |
| Specificity | Recognizes both human platelet-derived and recombinant TGF-beta. |
| Warning | For research use only. |
| Application Info | ELISA, FN, IHC, IHC-F, IHC-P (10 ug/ml), NT, WB |
| Application Data | Suitable for use in Western Blot, ELISA, Neutralization and Immunohistochemistry. Neutralization: Neutralizes TGF-beta activity in vitro in an inhibition assay of CCL/64 cell growth and the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of microinjection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo. Immunohistochemistry (formalin-fixed, paraffin-embedded, frozen): 1:1000. Has been used to demonstrate TGF-beta in ovine ovarian tissue and human breast carcinoma. As a consequence of the intense staining of the erythrocytes it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue. Western Blot: 5-20 ng/ml will allow visualization of 100 ng/lane of TGF-beta. Recognizes the dimeric (25kD) and monomeric (12.5kD) forms of TGF-beta under both non-reducing and reducing conditions respectively. |
| Storage Buffer | PBS, pH 7.0 |
| Gene Symbol | TGFB1 |
|---|---|
| Gene Full Name | Transforming growth factor beta-1 |
| Protein Name | Transforming growth factor beta-1 |
| Uniprot ID | P01137 |
| Protein Accession # | NP_000651.3 |
| Nucleotide Accession # | NM_000660.5 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "TGFB1 Antibody (OALA04428)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".
-
How long will it take to receive "TGFB1 Antibody (OALA04428)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
-
What buffer format is "TGFB1 Antibody (OALA04428)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "TGFB1 Antibody (OALA04428)"?
This target may also be called "" in publications.
-
What is the shipping cost for "TGFB1 Antibody (OALA04428)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "TGFB1 Antibody (OALA04428)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "TGFB1 Antibody (OALA04428)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "TGFB1 Antibody (OALA04428)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "TGFB1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "TGFB1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "TGFB1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "TGFB1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "TGFB1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "TGFB1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
