Catalog No: OKCD03943
Size:96WELLS
Price: $800.00
SKU
OKCD03943
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TGFA Chemi-Luminescent ELISA Kit (Human) (OKCD03943)

Datasheets/ManualsPrintable datasheet for TGFA Chemi-Luminescent ELISA Kit (Human) (OKCD03943)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)83-104%84-93%89-96%81-95%
EDTA Plasma (n=5)85-96%93-105%80-94%86-102%
Heparin Plasma (n=5)80-99%94-102%98-105%80-90%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Alpha (TGFa). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Alpha (TGFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Transforming Growth Factor Alpha (TGFa) level in the sample or standard.
ELISA Kit Range1.37-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)90-10194
EDTA Plasma (n=5)93-10197
Heparin Plasma (n=5)78-9985
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Alpha (TGFa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Alpha (TGFa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
TGFA Microplate96 Wells (12 x 8 Well strips)
TGFA Lyophilized Standard2
100X TGFA Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity2.74 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTGFA
Gene Full Nametransforming growth factor alpha
Alias Symbolsprotransforming growth factor alpha;TFGA;TGF-alpha.
NCBI Gene Id7039
Protein NameProtransforming growth factor alpha
Description of TargetTGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Uniprot IDP01135
Protein Accession #NP_001093161
Nucleotide Accession #NM_001099691
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