TFRC Antibody (OAEE00308)

Catalog No: OAEE00308

Size:100UG

Immunofluorescence staining of CD71 in human HeLa cell line using anti-CD71 (MEM-75; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Surface staining of NALM-6 human peripheral blood pre-B cell leukemia cell line with anti-CD71 (MEM-75) biotin; detection by Streptavidin-PE.
Total viable cells were used for analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: MEM-75
• Isotype: IgG1
• Host: Mouse
• Application: Flow cytometry|Immunocytochemistry|Immunoprecipitation
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: NALM-6 human pre-B cell line
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody MEM-75 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes.
  The antibody MEM-75 does not block binding of transferrin to the receptor.
  HLDA IV; WS Code A 45
  HLDA V; WS Code T T-165
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
• Reference: Beck Z, Balogh A, Kis A, Izsépi E, Cervenak L, László G, Bíró A, Liliom K, Mocsár G, Vámosi G, Füst G, Matko J: New cholesterol-specific antibodies remodel HIV-1 target cells' surface and inhibit their in vitro virus production. J Lipid Res. 2010 Feb;51(2):286-96.
  Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
  Graham RM, Reutens GM, Herbison CE, Delima RD, Chua AC, Olynyk JK, Trinder D: Transferrin receptor 2 mediates uptake of transferrin-bound and non-transferrin-bound iron. J Hepatol. 2008 Feb;48(2):327-34.
  Graham RM, Chua AC, Herbison CE, Olynyk JK, Trinder D: Liver iron transport. World J Gastroenterol. 2007 Sep 21;13(35):4725-36.
  Taketani S: Aquisition, mobilization and utilization of cellular iron and heme: endless findings and growing evidence of tight regulation. Tohoku J Exp Med. 2005 Apr;205(4):297-318.
  Rouault TA: How mammals acquire and distribute iron needed for oxygen-based metabolism. PLoS Biol. 2003 Dec;1(3):E79.

Target Info

• Gene Symbol: TFRC
• Gene Full Name: transferrin receptor
• Alias Symbols: CD71;IMD46;p90;T9;TFR;TFR1;TR;transferrin receptor protein 1;TRFR.
• NCBI Gene Id: 7037
• Protein Name: Transferrin receptor protein 1
• Description of Target: Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
• Uniprot ID: P02786
• Protein Accession #: NP_001121620.1; NP_003225.2
• Nucleotide Accession #: NM_001128148.1; NM_003234.2