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Catalog No: OKAG00251 (Formerly GWB-SKR251)
Size:12x8WELLS
Price: $475.00
SKU
OKAG00251
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
Product Format1 x 96-Well Plate or 12 x 8-Well Strips
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric 450 nm
ELISA Kit Duration4.5 hours
ELISA Kit PrincipleThe Aviva Human TFPI ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human TFPI concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human TFPI while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
ELISA Kit Range16-1000 pg/ml
ELISA Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Protein StandardLyophilized (5 ng)
Biotinylated Detection AntibodyLyophilized
400x Streptavidin-HRP30μl
Wash Buffer (15x)50 mL
Assay Diluent50 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Reconstitution and Storage2°C to 8°C
Sample TypeCell lysates, sera and plasma
Sensitivity16 pg/mL
SpecificityThe Human TFPI ELISA is capable of recognizing both recombinant and naturally produced Human TFPI proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: Coagulation Factor VII, Coagulation Factor XI, Coagulation Factor XIV, TFPI-2, Vitronectin, Coagulation Factor II, HDL, LDL, VLDL
• Murine: TFPI, TFPI-2
Assay InfoQuantitative Colorimentric Sandwich ELISA
Gene SymbolTFPI
Gene Full Nametissue factor pathway inhibitor
Alias Symbolsanti-convertin;EPI;extrinsic pathway inhibitor;LACI;Lipoprotein-associated coagulation inhibitor;TFI;TFPI1;tissue factor pathway inhibitor;tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor).
NCBI Gene Id7035
Protein NameP10646
Description of TargetTissue factor pathway inhibitor
Uniprot IDhttps://www.uniprot.org/uniprot/P10646
Protein Accession #NP_001027452.1
Nucleotide Accession #NM_001032281.2
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