Tff3 ELISA Kit (Mouse) (OKEH04680)

Catalog No: OKEH04680

Size:96T

Mouse Tff3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Tff3 ELISA Kit (Mouse) (OKEH04680) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tff3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Tff3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tff3 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Anti-Tff3 Microplate: 96 Wells (12 x 8 Well Strips)
  • Tff3 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • Tff3 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.156 ng/mL
• Specificity: Natural and recombinant Mouse Trefoil factor 3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Tff3
• Gene Full Name: trefoil factor 3, intestinal
• Alias Symbols: intestinal trefoil factor;IT;ITF;mIT;mITF;trefoil factor 3.
• NCBI Gene Id: 21786
• Protein Name: Q62395
• Description of Target: Trefoil factor 3
• Uniprot ID: https://www.uniprot.org/uniprot/Q62395
• Protein Accession #: NP_035705.1
• Nucleotide Accession #: NM_011575.2
• Protein Size (# AA): 81
• Molecular Weight: 9 KDa