TFF3 ELISA Kit (Bovine) (OKEH08403)

Catalog No: OKEH08403

Size:96T

Bovine TFF3 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology TFF3 ELISA Kit (OKEH08403) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-TFF3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TFF3 and incubated. The TFF3 found in the sample or standards competes with the biotinylated TFF3 for limited binding sites on the immobilized anti-TFF3 antibody. Excess unbound biotinylated TFF3 and sample or standard TFF3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TFF3 captured in the well and inversely proportional to the amount of TFF3 which was contained in the sample or standard.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.0% (n = 20)
  Mean Inter-assay CV%: <=7.5% (n = 20)
• ELISA Kit Component:
  • Anti-TFF3 Microplate: 96 Wells (12 x 8 Well strips)
  • TFF3 Lyophilized Standard: 2 x 100 ng
  • Sample Diluent: 1 x 20 mL
  • 100X TFF3-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
  Subcellular Location: Secreted Extracellular space Extracellular matrix Cytoplasm
  Unigene: Bt.56560
  SMR: A8YXX7
  String: 9913.ENSBTAP00000028349
  KEGG: bta:517889
  Pfam: PF00088
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.26ng/mL
• Specificity: Natural and recombinant Bovine Trefoil factor 3
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: TFF3
• Gene Full Name: trefoil factor 3
• Alias Symbols: intestinal trefoil factor;trefoil factor 3;trefoil factor 3 (intestinal).
• NCBI Gene Id: 517889
• Protein Name: A8YXX7
• Description of Target: Trefoil factor 3
• Uniprot ID: https://www.uniprot.org/uniprot/A8YXX7
• Protein Accession #: NP_001107981.1
• Nucleotide Accession #: NM_001114509.2