TFF1 Polyclonal Antibody (OABB00641)

Catalog No: OABB00641 (Formerly GWB-BBP564)

Size:100UG

IHC(P): Human Gastric Cancer Tissue

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: TFF1(Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera directed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.
  Background: TFF1(Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera directed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls.This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
• Reference: 1. Chutipongtanate, S., Nakagawa, Y., Sritippayawan, S., Pittayamateekul, J., Parichatikanond, P.,
  Westley, B. R., May, F. E. B., Malasit, P., Thongboonkerd, V.Identification of human urinary trefoil
  factor 1 as a novel calcium oxalate crystal growth inhibitor.J. Clin. Invest. 115: 3613-3622, 2005.
  2. Hanby, A. M., Poulsom, R., Singh, S., Elia, G., Jeffery, R. E., Wright, N. A.Spasmolytic polypeptide
  is a major antral peptide: distribution of the trefoil peptides human spasmolytic polypeptide and pS2
  in the stomach.Gastroenterology 105: 1110-1116, 1993.
  3. Roberts, M., Wallace, J., Jeltsch, J.-M., Berry, M.The 5-prime flanking region of the human pS2
  gene mediates its transcriptional activation by estrogen in MCF-7 cells.Biochem. Biophys. Res.
  Commun. 151: 306-313, 1988
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Trefoil factor 1(TFF1) detection. Tested with WB, IHC-P in Human.

Target Info

• Gene Symbol: TFF1
• Gene Full Name: trefoil factor 1
• Alias Symbols: BCEI;breast cancer estrogen-inducible protein;breast cancer estrogen-inducible sequence;D21S21;gastrointestinal trefoil protein pS2;HP1.A;HPS2;pNR-2;polypeptide P1.A;protein pS2;pS2;trefoil factor 1.
• NCBI Gene Id: 7031
• Protein Name: Trefoil factor 1
• Description of Target: Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.
• Uniprot ID: P04155
• Molecular Weight: 9150 MW