TFE3 Antibody - N-terminal region (OAAB13846)

Catalog No: OAAB13846

Size:400ul

TFE3 Antibody (N-term) (Cat. #OAAB13846) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TFE3 Antibody detected the TFE3 protein (arrow).

TFE3 Antibody (N-term) (Cat. #OAAB13846) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the TFE3 antibody detected the TFE3 protein (arrow).

Anti-TFE3 Antibody (N-term)at 1:2000 dilution + HepG2 whole cell lysates Lysates/proteins at 20 ug per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 62 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of Hela cell stained with TFE3 Antibody (N-term)(Cat#OAAB13846). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFE3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TFE3 immunoreactivity is localized to nucleus and Cytoplasm significantly.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: IF, WB
• Additional Information: Function: Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site
  Cellular Location: Nucleus.
  Tissue Location: Ubiquitous in fetal and adult tissues.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This TFE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TFE3.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Intended Use: TFE3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:2000
  WB: 1:1000
  IF: 1:10~50

Target Info

• Gene Symbol: TFE3
• Alias Symbols: TFEA, RCCP2, RCCX1, bHLHe33
• NCBI Gene Id: 7030
• Protein Name: Transcription factor E3
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: P19532
• Protein Accession #: NP_006512.2
• Molecular Weight: 61521 Da