TFDP1 Antibody (OAAF01778)

Catalog No: OAAF01778 (Formerly GWB-ASD053)

Size:100 ug

Western blot analysis of extracts from HeLa cells, using DP-1 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using DP-1 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using DP-1 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human DP-1.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: DP-1 antibody detects endogenous levels of total DP-1 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:5000
• Reference: K Helin, Genes & Dev., Oct 1993; 7: 1850 - 1861.
  B Shan, Cell Growth Differ., Jun 1996; 7: 689.
  Hironori Ishida, J. Biol. Chem., Jul 2005; 280: 24642 - 24648.
  Wing Y. Chang, J. Biol. Chem., Dec 2004; 279: 51343 - 51353.

Target Info

• Gene Symbol: TFDP1
• Gene Full Name: transcription factor Dp-1
• Alias Symbols: DILC;down-regulated in liver cancer stem cells;Dp-1;DP1;DRTF1;DRTF1-polypeptide 1;E2F dimerization partner 1;E2F-related transcription factor;transcription factor Dp-1.
• NCBI Gene Id: 7027
• Protein Name: Transcription factor Dp-1
• Description of Target: Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication (PubMed:8405995, PubMed:7739537). The E2F1:DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters (PubMed:20176812).
• Uniprot ID: Q14186
• Protein Accession #: NP_009042.1
• Molecular Weight: 45 kDa