TFAP2C Antibody (OAEB00838)

Catalog No: OAEB00838 (Formerly GWB-7784EA)

Size:100UG

OAEB00838 (4ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC
• Additional Information: Amino Acids: 450 aa
  Reactivity Info: Tested: Human; Expected: Human, Rat, Mouse, Dog
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: TFAP2C
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 90%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 90%; Pig: 90%; Rabbit: 100%; Rat: 91%
• Peptide Sequence: C-NKTLEKMEKHRK
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: Antibody detection limit dilution 1:128000.
  Immunohistochemistry: In paraffin embedded Human Skin shows nuclear staining of keratinocytes. Recommended concentration, 2-3ug/ml.
  Western Blot: WB experiments gave bands at approx 75kDa and 22kDa in Human Placenta and Ovary lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 49.2kDa according to NP_003213.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
• Reference: Hoei-Hansen CE, Rajpert-De Meyts E, Carlsen E, Almstrup K, Leffers H, Skakkebaek NE.
  Fast-track Article: A subfertile patient diagnosed with testicular carcinoma in situ by immunocytological staining for AP-2{gamma} in semen samples: Case report.
  Hum Reprod. 2005 Jan 13; [Epub ahead of print] PMID: 15650041

Target Info

• Gene Symbol: TFAP2C
• Gene Full Name: transcription factor AP-2 gamma
• Alias Symbols: activating enhancer-binding protein 2 gamma;AP2-GAMMA;ERF1;estrogen receptor factor 1;hAP-2g;TFAP2G;transcription factor AP-2 gamma;transcription factor ERF-1.
• NCBI Gene Id: 7022
• Protein Name: Transcription factor AP-2 gamma
• Uniprot ID: Q92754
• Protein Accession #: NP_003213.1
• Molecular Weight: 49.2