TFAP2B Antibody (OAGA04820)

Catalog No: OAGA04820

Size:100 ul

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AP2 beta antibody diluted at 1:500.

Sample (30 ug of whole cell lysate)
A: MCF-7
10% SDS PAGE
diluted at 1:1000

AP2 beta antibody immunoprecipitates AP2 beta protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. 30 ug MCF-7 whole cell extract
B. Control with 4 ug of preimmune Rabbit IgG
C. Immunoprecipitation of AP2 beta protein by 4 ug AP2 beta antibody
7.5 % SDS-PAGE
The immunoprecipitated AP2 beta protein was detected by AP2 beta antibody diluted at 1:1000.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]

Product Info

• Predicted Species Reactivity: Human, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IP, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human AP2 beta. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.95 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: TFAP2B
• Gene Full Name: transcription factor AP-2 beta
• Alias Symbols: PDA2, AP-2B, AP2-B, AP-2beta
• NCBI Gene Id: 7021
• Protein Name: transcription factor AP-2-beta
• Description of Target: This gene encodes a member of the AP-2 family of transcription factors. AP-2 proteins form homo- or hetero-dimers with other AP-2 family members and bind specific DNA sequences. They are thought to stimulate cell proliferation and suppress terminal differentiation of specific cell types during embryonic development. Specific AP-2 family members differ in their expression patterns and binding affinity for different promoters. This protein functions as both a transcriptional activator and repressor. Mutations in this gene result in autosomal dominant Char syndrome, suggesting that this gene functions in the differentiation of neural crest cell derivatives.
• Uniprot ID: Q92481
• Protein Accession #: NP_003212.2
• Nucleotide Accession #: NM_003221.3