TFAP2A Antibody (OAGA04360)

Catalog No: OAGA04360

Size:100 ul

Sample (30 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
diluted at 1:1000

AP2 alpha antibody detects AP2 alpha protein by western blot analysis. Whole cell extracts (30 ug) was separated by 10 % SDS-PAGE, and blotted with AP2 alpha antibody diluted by 1:1000

AP2 alpha antibody detects AP2 alpha protein by western blot analysis. Whole cell extracts (30 ug) was separated by 10 % SDS-PAGE, and blotted with AP2 alpha antibody diluted by 1:1000, and developed with Trident femto Western HRP Substrate .

AP2 alpha antibody immunoprecipitates AP2 alpha protein in IP experiments.
IP samples: HeLa nuclear extract
A. 30 ug HeLa nuclear extract
B. Control with 4 ug of preimmune Rabbit IgG
C. Immunoprecipitation of AP2 alpha protein by 4 ug AP2 alpha antibody
10 % SDS-PAGE
The immunoprecipitated AP2 alpha protein was detected by AP2 alpha antibody diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IP, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human AP2 alpha. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.64 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: TFAP2A
• Gene Full Name: transcription factor AP-2 alpha
• Alias Symbols: AP-2, BOFS, AP2TF, TFAP2, AP-2alpha
• NCBI Gene Id: 7020
• Protein Name: transcription factor AP-2-alpha
• Description of Target: The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P05549
• Protein Accession #: NP_001027451.1
• Nucleotide Accession #: NM_001032280.2