TFAM Antibody - C-terminal region (OABB01082)

Catalog No: OABB01082

Size:100UG

Anti-mtTFA antibody, OABB01082, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: JURKAT Cell Lysate
Lane 3: 293T Cell Lysate
Lane 4: Rabbit igG(55kD)
Lane 5: A431 Cell Lysate
Lane 6: RAJI Cell Lysate
Lane 7: CEM Cell Lysate
Lane 8: HL-60 Cell Lysate
Lane 9: HUT Cell Lysate

Anti-mtTFA antibody, OABB01082, IHC(F)
IHC(F): Human Placenta Tissue

Anti-mtTFA antibody, OABB01082, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

Anti-mtTFA antibody, OABB01082, ICC
ICC: HELA Cell

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunocytochemistry, Immunohistochemistry, Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: TFAM(Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a 162-amino acid protein that activates transcription of each mitochondrial DNA(mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al.(1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott(2007) stated that the sequences mapped to chromosomes 7p(TCF6L1) and 11q(MTTF1, or TCF6L3) are pseudogenes. Larsson et al.(1997) mapped the mouse mitochondrial transcription factor A gene(Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human mtTFA (227-246aa RKDLLRRTIKKQRKYGAEEC).
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
  Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Human
  Immunocytochemistry : 0.5-1 ug/ml: Human
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Transcription factor A, mitochondrial(TFAM) detection. Tested with WB, IHC-P, IHC-F, ICC in Human.

Target Info

• Gene Symbol: TFAM
• Gene Full Name: transcription factor A, mitochondrial
• Alias Symbols: mitochondrial transcription factor 1;mitochondrial transcription factor A;MTDPS15;MTTF1;MTTFA;TCF6;TCF6L1;TCF6L2;TCF6L3;transcription factor 6;transcription factor 6-like 1;Transcription factor 6-like 2;transcription factor 6-like 2 (mitochondrial transcription factor);transcription factor 6-like 3;transcription factor A, mitochondrial.
• NCBI Gene Id: 7019
• Protein Name: Q00059
• Description of Target: Transcription factor A, mitochondrial
• Uniprot ID: https://www.uniprot.org/uniprot/Q00059
• Molecular Weight: 29097 MW