TET3 Antibody - C-terminal region (OAGA06748)

Catalog No: OAGA06748

Size:100 ul

TET3 antibody [C3], C-term detects TET3 protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug whole cell lysate/extract of Flag-human TET3 transfected 293T cells
5 % SDS-PAGE
TET3 antibody [C3], C-term dilution: 1:5000

TET3 antibody [C3], C-term detects TET3 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
TET3 antibody [C3], C-term dilution: 1:500.

TET3 antibody [C3], C-term detects TET3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET3 protein stained by TET3 antibody [C3], C-term diluted at 1:400.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

TET3 antibody immunoprecipitates TET3 protein in IP experiments. IP Sample: cell lysate/extract of TET3 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of TET3 by 2 ug of TET3 antibody 5% SDS-PAGE The immunoprecipitated TET3 protein was detected by TET3 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human, Mouse, Bovine, Xenopus laevis
• Product Format: Liquid 1XPBS, 20% glycerol (pH 7) with 0.01% Thimerosal added as a preservative
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: CHIP, ICC, IF, IHC-FFPE, IP, WB, IHC (whole mount)
  Positive Controls: Flag-human TET3 transfected 293T
• Reconstitution and Storage: Store as a concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze/thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human TET3. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: ICC/IF: 1:100~1000
  IHC-FFPE: 1:100~1000
  IP: 1:500~1000
  WB: 1:1000~10000
  IHC (whole mount): Assay-dependent dilution
  CHIP: Assay-dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the user.

Target Info

• Gene Symbol: TET3
• Gene Full Name: tet methylcytosine dioxygenase 3
• Alias Symbols: BEFAHRS, hCG_40738
• NCBI Gene Id: 200424
• Protein Name: methylcytosine dioxygenase TET3
• Description of Target: Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).[supplied by OMIM, Nov 2010]
• Uniprot ID: O43151
• Protein Accession #: NP_001274420.1
• Nucleotide Accession #: NM_001287491.1
• Molecular Weight: 179 kDa