TERF1 ELISA Kit (Human) (OKCD00350)

Catalog No: OKCD00350 (Formerly GWB-KBBUH5)

Size:96WELLS

Human TERF1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Telomeric Repeat Binding Factor 1 (TERF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Telomeric Repeat Binding Factor 1 (TERF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Telomeric Repeat Binding Factor 1 (TERF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Telomeric Repeat Binding Factor 1 (TERF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Telomeric Repeat Binding Factor 1 (TERF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Telomeric Repeat Binding Factor 1 (TERF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TERF1 Microplate: 96 Wells (12 x 8 Well Strips)
  • TERF1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated TERF1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Telomeric Repeat Binding Factor 1 (TERF1). No significant cross-reactivity or interference between Telomeric Repeat Binding Factor 1 (TERF1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TERF1
• Gene Full Name: telomeric repeat binding factor 1
• Alias Symbols: hTRF1-AS;NIMA-interacting protein 2;PIN2;telomeric protein Pin2/TRF1;telomeric repeat binding factor (NIMA-interacting) 1;telomeric repeat-binding factor 1;TRBF1;TRF;TRF1;TTAGGG repeat-binding factor 1;t-TRF1.
• NCBI Gene Id: 7013
• Protein Name: Telomeric repeat-binding factor 1
• Description of Target: Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
• Uniprot ID: P54274
• Protein Accession #: NP_003209
• Nucleotide Accession #: NM_003218.3
• Protein Size (# AA): 439