TEK/TIE2 ELISA Kit (Mouse) (OKBB00481)

Catalog No: OKBB00481

Size:96 Wells

Mouse TEK/TIE2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse TEK ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Monoclonal antibody from rat specific for TEK has been precoated onto 96-well plates. Standards(NS0, A23-K744) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TEK is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse TEK amount of sample captured in plate.
• ELISA Kit Range: 125 pg/ml - 8,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	454	1225	4837	632	1586	5124
  SD	30.9	77.2	285.4	46.14	103.1	312.6
  CV%	6.8	6.3	5.9	7.3	6.5	6.1

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse TIE2 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse TIE2 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse TIE2 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, tissue homogenates, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse TEK
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A23-K744
• Description: For quantitative detection of mouse TEK in cell culture supernatants, serum and plasma(heparin, EDTA).

Target Info

• Gene Symbol: TEK
• Gene Full Name: TEK tyrosine kinase, endothelial
• Alias Symbols: Angiopoietin-1 receptor, CD202b, CD202b antigen, EC 2.7.10.1, hTIE2, p140 TEK, soluble TIE2, variant 1, soluble TIE2 variant 2, Tek, TEK tyrosine kinase, endothelial, TIE 2, TIE2_HUMAN, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE 2, Tyrosine-protein kinase receptor TIE-2, venous malformations, multiple cutaneous and mucosal, VMCM, VMCM1
• NCBI Gene Id: 21687
• Protein Name: Angiopoietin-1 receptor
• Description of Target: Angiopoietin-1 receptor, also called TEK or TIE2 is a protein that in humans is encoded by the TEK gene. This gene is mapped to 9p21.2. The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations, the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.
• Uniprot ID: Q02858
• Protein Accession #: NP_001277478.1
• Nucleotide Accession #: NM_001290549.1