TCRB Antibody : APC (OATA00096)

Catalog No: OATA00096

C57Bl/6 splenocytes were stained with 0.125 ug APC Anti-Mouse TCR beta (OATA00096) (solid line) or 0.125 ug APC Armenian hamster IgG isotype control (dashed line).

Product Info

• Tested Species Reactivity: Mouse
• Product Format: Liquid 10mM NaH₂PO₄, 150mM NaCl, 0.09% sodium azide and 0.1% gelatin (pH 7.2)
• Clonality: Monoclonal
• Clone: H57-597
• Isotype: Armenian Hamster IgG
• Host: Hamster
• Conjugation: APC: Allophycocyanin
• Application: FC
• Reconstitution and Storage: It is recommended to store the product undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Purification: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography.
• Concentration: 0.2 mg/ml
• Specificity: The H57-597 antibody is specific for the beta chain of the mouse T cell Receptor (TCR). The antibody does not cross-react with cells expressing the gamma-delta TCR.
• Application Info: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system.
• Preparation: The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
• Reference:

  Berent-Maoz B, Montecino-Rodriguez E, Signer RAJ, and Dorshkind K. 2012. Blood. 199:5715-5721. (Flow cytometry)

  Wang D, Qin H, Du W, Shen Y-W, Lee W-H, Riggs AD, and Liu C-P. 2012. Proc. Natl. Acad. Sci. 109:9493-9498. (in vitro induction of apoptosis)

  O"™Brian RL, Taylor MA, Hartley J, Nuhsbaum T, Dugan S, Lahmers K, Aydintug MK, Wands JM, Roark CL, and Born WK. 2009. Invest. Ophthalmol. Vis. Sci. 50: 3266-3274. (Immunofluorescence microscopy - OCT embedded frozen tissue)

  Matei IR, Gladdy RA, Nutter LMJ, Canty A, Guidos CJ, and Danska JS. 2007. Blood. 109:1887-1896. (Immunoprecipitation)

  Harada N, Shimada M, Okano S, Suehiro T, Soejima Y, Tomita Y, and Maehara Y. 2004. J. Immunol. 173:6635-6644. (in vivo T cell depletion)

  Kubo RT, Born W, Kappler JW, Marrack P, and Pigeon M. 1989. J. Immunol. 142: 2736-2742. (Origination of clone, Immunoprecipitation, in vitro activation)

Target Info

• Gene Symbol: TCRB
• Gene Full Name: T cell receptor beta chain
• Alias Symbols: Ti, Tib, TCRbe, TCRbeta
• NCBI Gene Id: 21577
• Description of Target: This cell surface protein combines with a second protein chain (alpha chain) to form the alpha-beta TCR that is expressed by NK1.1+ thymocytes, NKT cells, and the majority of peripheral T cells. A small number of T cells may express an alternative heteromer of gamma and delta protein chains, known as the gamma-delta TCR. These receptors participate in a complex with CD3, and with the co-receptors CD4 or CD8, to recognize and respond to antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. The H57-597 antibody is used as a phenotypic marker for T cells expressing the alpha-beta TCR. It is also widely used to cross-link surface TCR and thereby mimic TCR-mediated cell activation or induction of apoptosis.