TCP1-alpha Antibody : Biotin (OASE00325-Biotin)

Catalog No: OASE00325-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Rabbit, Guinea pig, Hamster, Cow, Dog, Pig, Saccharomyces cerevisiae, Caenorhabditis elegans, Fruit fly, Monkey
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: 91a
• Isotype: IgG2a
• Host: Rat
• Conjugation: Biotin
• Application: WB, IP, FC, IHC
• Additional Information: Background Info: Detects a 60kDa protein corresponding to the molecular weight of TCP1 alpha; also detects a molecular mass band of ~92kDa. Cross reactivity with human Hsp60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.
  Scientific Background: T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60,Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.
  
  Certificate of Analysis: 1ug/mL was sufficient for detection of TCP1 alpha in 20 ug of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:1000 (WB)
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.1% sodium azide

Target Info

• Gene Symbol: Tcp1
• Gene Full Name: t-complex protein 1
• Alias Symbols: TR, p6, CCT, Tp6, c-c, p63, Cct1, Ccta, TRic, Tcp-, Tp63, Tcp-1, c-cpn, AI528772
• NCBI Gene Id: 21454
• Uniprot ID: P11983
• Protein Accession #: NP_038714.2