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Catalog No: OKEH02274
Size:96T
Price: $625.00
SKU
OKEH02274
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TCN2 ELISA Kit (Rat) (OKEH02274) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat TCN2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TCN2 and incubated. The TCN2 found in the sample or standards competes with the biotinylated TCN2 for limited binding sites on the immobilized anti-Rat TCN2 antibody. Excess unbound biotinylated TCN2 and sample or standard TCN2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TCN2 captured in the well and inversely proportional to the amount of TCN2 which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 109%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 9.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-TCN2 Microplate96 Wells (12 x 8 Well strips)
TCN2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X TCN2 Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.178 ng/mL
SpecificityNatural and recombinant Rat Transcobalamin-2
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolTCN2
Gene Full Nametranscobalamin 2
Alias SymbolsTranscobalamin II.
NCBI Gene Id64365
Protein NameQ9R0D6
Description of TargetTranscobalamin-2
Uniprot IDhttps://www.uniprot.org/uniprot/Q9R0D6
Protein Accession #NP_071979.2
Nucleotide Accession #NM_022534.2
Protein Size (# AA)427
Molecular Weight48 kDa
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