Catalog No: OKCD00507 (Formerly GWB-KBBLF9)
Size:96WELLS
Price: $675.00
SKU
OKCD00507
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Datasheets/ManualsPrintable datasheet for TCHH ELISA Kit (Human) (OKCD00507)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trichohyalin (TCHH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Trichohyalin (TCHH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Trichohyalin (TCHH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Trichohyalin (TCHH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Trichohyalin (TCHH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Trichohyalin (TCHH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-TCHH Microplate96 Wells (12 x 8 Well Strips)
TCHH Lyophilized Standard2 x 10 ng
100X Biotinylated TCHH Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.057 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Trichohyalin (TCHH). No significant cross-reactivity or interference between Trichohyalin (TCHH) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTCHH
Gene Full Nametrichohyalin
Alias SymbolsTHH;THL;TRHY;trichohyalin;UHS3.
NCBI Gene Id7062
Protein NameTrichohyalin
Description of TargetIntermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation.
Uniprot IDQ07283
Protein Accession #NP_009044
Nucleotide Accession #NM_007113.3
Protein Size (# AA)1943
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