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Catalog No: OCOA15347
Size:20UG
Price: $275.00
SKU
OCOA15347
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TCF4 Protein Lysate (OCOA15347)

Datasheets/ManualsPrintable datasheet for TCF4 Protein Lysate (OCOA15347)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionTCF4 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolTCF4
Gene Full Nametranscription factor 4
Alias SymbolsbHLHb19;CDG2T;class B basic helix-loop-helix protein 19;E2-2;FECD3;immunoglobulin transcription factor 2;ITF2;ITF-2;PTHS;SEF2;SEF-2;SEF2-1;SEF2-1A;SEF2-1B;SEF2-1D;SL3-3 enhancer factor 2;TCF-4;transcription factor 4.
NCBI Gene Id6925
Protein NameB3KVA4, NM_001083962, NP_001077431, P15884
Description of TargetcDNA FLJ16304 fis, clone PUAEN2003408, highly similar to TRANSCRIPTION FACTOR 4, Homo sapiens transcription factor 4 (TCF4), transcript variant 1, mRNA, Transcription factor 4, transcription factor 4 isoform a [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P15884
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001077431
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001083962
Molecular Weight71.6 kDa
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