Catalog No: OCOA18967
Size:20UG
Price: $169.00
SKU
OCOA18967
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TCF3 Protein Lysate (OCOA18967)

Datasheets/ManualsPrintable datasheet for TCF3 Protein Lysate (OCOA18967)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionTCF3 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolTCF3
Gene Full Nametranscription factor 3
Alias SymbolsAGM8;bHLHb21;class B basic helix-loop-helix protein 21;E2A;E2A-HLF fusion transcript protein;E47;helix-loop-helix protein HE47;Immunoglobulin enhancer-binding factor E12/E47;immunoglobulin transcription factor 1;ITF1;kappa-E2-binding factor;negative vitamin D response element-binding protein;NOL1-TCF3 fusion;p75;TCF-3;Transcription factor 3;transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47);transcription factor 3 variant 3;transcription factor E2-alpha;transcription factor ITF-1;VDIR;VDR interacting repressor;vitamin D receptor-interacting repressor.
NCBI Gene Id6929
Protein NameNM_001136139, NP_001129611, P15923
Description of TargetHomo sapiens transcription factor 3 (TCF3), transcript variant 2, mRNA, Transcription factor E2-alpha, transcription factor E2-alpha isoform E47 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P15923
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001129611
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001136139
Molecular Weight67.1 kDa
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