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Catalog No: OKEH02408
Size:96T
Price: $625.00
SKU
OKEH02408
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TCF3 ELISA Kit (Human) : 96 Wells (OKEH02408)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Comp
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TCF3 ELISA Kit (Human) (OKEH02408) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human TCF3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TCF3 and incubated. The TCF3 found in the sample or standards competes with the biotinylated TCF3 for limited binding sites on the immobilized anti-Human TCF3 antibody. Excess unbound biotinylated TCF3 and sample or standard TCF3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TCF3 captured in the well and inversely proportional to the amount of TCF3 which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 8.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-TCF3 Microplate96 Wells (12 x 8 Well strips)
TCF3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X CA3-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.19 ng/mL
SpecificityNatural and recombinant Human Transcription factor E2-alpha
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolTCF3
Gene Full Nametranscription factor 3
Alias SymbolsAGM8;bHLHb21;class B basic helix-loop-helix protein 21;E2A;E2A-HLF fusion transcript protein;E47;helix-loop-helix protein HE47;Immunoglobulin enhancer-binding factor E12/E47;immunoglobulin transcription factor 1;ITF1;kappa-E2-binding factor;negative vitamin D response element-binding protein;NOL1-TCF3 fusion;p75;TCF-3;Transcription factor 3;transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47);transcription factor 3 variant 3;transcription factor E2-alpha;transcription factor ITF-1;VDIR;VDR interacting repressor;vitamin D receptor-interacting repressor.
NCBI Gene Id6929
Protein NameP15923
Description of TargetTranscription factor E2-alpha
Uniprot IDhttps://www.uniprot.org/uniprot/P15923
Protein Accession #NP_001129611.1
Nucleotide Accession #NM_001136139.2
Protein Size (# AA)654
Molecular Weight68 kDa
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