Search Antibody, Protein, and ELISA Kit Solutions

TBX18 Antibody - C-terminal region (ARP37832_P050)

100 ul
$319.00
In Stock
Request Bulk Order Quote

Conjugation Options

ARP37832_P050-FITC Conjugated

ARP37832_P050-HRP Conjugated

ARP37832_P050-Biotin Conjugated

Tested Species Reactivity:
Human, Mouse
Predicted Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Clonality:
Polyclonal
Host:
Rabbit
Application:
IHC, WB
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Replacement Item:
This antibody may replace item sc-130428 from Santa Cruz Biotechnology.
Immunogen:
The immunogen is a synthetic peptide directed towards the C terminal region of human TBX18
Purification:
Affinity Purified
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 92%
Complete computational species homology data:
Anti-TBX18 (ARP37832_P050)
Peptide Sequence:
Synthetic peptide located within the following region: NQTHQGSYNTFRLHSPCALYGYNFSTSPKLAASPEKIVSSQGSFLGSSPS
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Blocking Peptide:
For anti-TBX18 (ARP37832_P050) antibody is Catalog # AAP37832 (Previous Catalog # AAPP09062)
Datasheets/Manuals:
Printable datasheet for anti-TBX18 (ARP37832_P050) antibody
Gene Symbol:
TBX18
Official Gene Full Name:
T-box 18
Alias Symbols:
-
NCBI Gene Id:
9096
Protein Name:
T-box transcription factor TBX18
Description of Target:
TBX18 is a probable transcriptional regulator involved in developmental processes.
Swissprot Id:
O95935
Protein Accession #:
XP_943361
Nucleotide Accession #:
XM_938268
Protein Size (# AA):
607
Molecular Weight:
65kDa
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express TBX18.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express TBX18.

Product Reviews

Average Rating:
1 review
5 star
0
4 star
0
3 star
1
2 star
0
1 star
0
  • Date - Newest First
  • Date - Newest First
  • Date - Latest First
  • Highest Rated
  • Lowest Rated
  • Most Helpful
  • Ownership

1 Item(s)

275/10/2019 21:35
  • Overall Experience:
  • Quality:
Zebrafish embryos, heart region in IHC

Submitted by:
Jie Ren
UCSD School Of Medicine

TBX18

" We did Tbx18 antibody staining in 24 hpf zebrafish embryos carrying the cmlc2:GFP transgene, which labels the cardiomyocytes of the heart. I do see some nuclear staining near the inflow tract of the heart (pointed out with arrows), but I'm not sure whether it's specific or nonspecific staining. We need to further validate what these cells are to make sure whether it's specific staining."

1. Species and tissue/cell type used: Zebrafish embryos, heart region.
2. Fixation method: 4% PFA overnight at 4C.
3. Primary antibody dilution: 1:100
4. Secondary antibody and dilution: 1:250
5. Stain/counterstain: Nuclear staining
6. Protocol:
1. Fix dechorionated embryos in 4% PFA overnight at 4C.
2. Wash 3x5 min with PBSTw.
3. Permeabilize embryos in 0.5% Triton in PBSTw for 2hrs at RT.
4. Wash 3x5min with PBSTw.
5. Block in 2mg/ml BSA in PBSTw for at least for 1hr at RT.
6. Incubate overnight at 4C in PBSTw + 2mg/ml BSA + Tbx18 antibody (1:100).
7. Wash 3x 5min with PBSTw.
8. Incubate in PBSTw + secondary antibody (goat anti rabbit 555, 1:250) for 2hrs at RT.
9. Wash 3x 5min with PBSTw.

0
0
Reply
Show more comments (-2) Hide comments

1 Item(s)

What kind of abuse are you reporting?
    Please, wait...