TBCK Antibody (OAGA00921)

Catalog No: OAGA00921

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Hep G2
B: Molt-4
7.5% SDS PAGE
OAGA00921 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
OAGA00921 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using TBCK(OAGA00921) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TBCK(OAGA00921) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
  Positive Controls: HepG2, Molt-4, NIH-3T3, JC, BCL-1
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 457 and 694 of TBCK (Uniprot ID#Q8TEA7)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.41 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: TBCK
• Gene Full Name: TBC domain-containing protein kinase-like protein
• Alias Symbols: TBCKL, IHPRF3, HSPC302
• NCBI Gene Id: 93627
• Protein Name: TBC domain-containing protein kinase-like protein
• Uniprot ID: Q8TEA7
• Protein Accession #: NP_001156907.1
• Molecular Weight: 94 kDa