TARP Gamma2/4/8 (Stargazin) Antibody : HRP (OASE00270-HRP)

Catalog No: OASE00270-HRP

Size:100ug

Product Info

• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Monoclonal
• Clone: S245-36
• Isotype: IgG2A
• Host: Mouse
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB, ICC
• Additional Information: Background Info: Detects ~35-55kDa. Does not cross-react with TARPGamma3.
  Scientific Background: Stargazin belongs to a group called voltage gated calcium channels (VGCCs) which are present in most excitable cells. Stargazin is thought to stabilize the calcium channel in an inactivated (closed) state.
  There are five high voltage activated calcium channel types (L, N, P, Q, and R) and one low voltage activated channel type (T). Each of these channels exists as a heteromultimer of a1, b, a2/ d and g subunits with the voltage activated calcium channel function carried by the a subunits. VGCCs exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival.
  Certificate of Analysis: 1 ug/mL was sufficient for detection of TARP Gamma2/4/8 in 20ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Fusion protein amino acids 203-323 (Cytoplasmic C-terminus) of rat TARPGamma2, accession number Q71RJ2
• Purification: Protein G Purified
• Concentration: 0.6-0.7 mg/ml
• Dilution: 1ug/ml WB
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Gill M.B., et al. (2011) J Neurosci. 31(18): 6928-6938.
  2. Yu L. (2011) Neuroscience. 178: 13-20.
  3. Steinmetz C.C., Turrigiano G.G. (2010) J Neurosci. 30: 14685-14690.
  4. Kato. A.S. et al. (2007) J Neurosci. 27: 4969-4977.
  5. Letts V.A., et al. (1998) Nat Genet. 19: 340-34

Target Info

• Gene Symbol: Cacng2
• Gene Full Name: calcium channel, voltage-dependent, gamma subunit 2
• Alias Symbols: Ipr328
• NCBI Gene Id: 84347
• Uniprot ID: Q71RJ2
• Protein Accession #: NP_445803.1